Pando Bioscience, AI enzyme design
Product 02

PD-Phi29

PD-Phi29 is an engineered version of the wild-type phage phi29 polymerase.

  • 1Increased activity
  • 2Increased thermostability
  • 3Reduced error rate

PD-Phi29 is our family of engineered wt-Phi29 polymerases. It delivers increased activity and increased thermostability.

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PD-Phi29
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PD-Phi29 engineered DNA polymerase activity and thermostability versus the market best
Figure 1. 100pg of cow genomic DNA was amplified using PD-phi29. The results were compared to the current market best and quantified using a dsDNA dye.
PD-Phi29 DNA polymerase processivity on genomic DNA measured on an Agilent TapeStation
Figure 2. Both enzymes were used to amplify 1pg of genomic DNA and measured on the Agilent TapeStation. The ladder is shown on the bottom, with two ladder-peaks marked on the x-axis.
PD-Phi29 DNA polymerase error rate in Oxford Nanopore full-plasmid sequencing
Figure 3. 1ng of DNA was amplified using PD-phi29 or the market leader, and the resulting MDA product was sequenced using Oxford Nanopore. Error rates were measured per-sequenced bp from high-quality reads.
Pando PD-Phi29 DNA polymerase enzyme vial supplied by Fortis Life Sciences
Figure 4. Product photo of Pando-engineered PD-Phi29 DNA polymerase supplied by Fortis Life Sciences.
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