Pando Bioscience
Products

High-performance enzymes

Off-the-shelf enzymes — starting with our Phi29 polymerase family for amplification and sequencing.

Product 01

Saiyan-Phi29

Saiyan-Phi29 series are engineered version of newly discovered Phi29-like enzymes, not an engineered or modified variant of an existing polymerase.

  • 1High fidelity
  • 2High processivity
  • 3High thermostability

Saiyan-Phi29 is our family of newly discovered Phi29-like polymerases, available as Saiyan-Phi1 and Saiyan-Phi2. It delivers high-fidelity, highly processive strand-displacement amplification, suited to whole-genome amplification and isothermal workflows.

Catalog
Saiyan-Phi1, Saiyan-Phi2
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Fidelity
Fidelity. Pando’s enzymes show higher fidelity than commercially available enzymes.
Chimeras
Chimeras. Pando’s enzymes produce far fewer chimeras than the market leader.
Processivity
Processivity. Pando’s hits produce longer DNA fragments than the market leader — 10 pg genomic DNA amplified with 1× diluted enzyme, fragment length on an Agilent TapeStation.
Activity
Activity. Pando’s discovered enzymes have reaction rates comparable to the market leader — similar enzyme concentrations amplifying 10 ng pUC19, dsDNA measured in real time (Qubit BR).
Product 02

PD-Phi29

PD-Phi29 is an engineered version of the wild-type phage phi29 polymerase.

  • 1Increased activity
  • 2Increased thermostability
  • 3Reduced error rate

PD-Phi29 is our family of engineered wt-Phi29 polymerases. It delivers increased activity and increased thermostability.

Catalog
PD-Phi29
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Increased activity and thermostability
Figure 1. 100pg of cow genomic DNA was amplified using PD-phi29. The results were compared to the current market best and quantified using a dsDNA dye.
High processivity
Figure 2. Both enzymes were used to amplify 1pg of genomic DNA and measured on the Agilent TapeStation. The ladder is shown on the bottom, with two ladder-peaks marked on the x-axis.
Higher success rate in full-plasmid sequencing
Figure 3. 1ng of DNA was amplified using PD-phi29 or the market leader, and the resulting MDA product was sequenced using Oxford Nanopore. Error rates were measured per-sequenced bp from high-quality reads.
Commercial-ready PD-Phi29
Figure 4. Product photo of Pando-engineered PD-Phi29 DNA polymerase supplied by Fortis Life Sciences.
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Proprietary enzymes for molecular biology applications

Interested in distributing Pando products or purchasing? Reach out and we will help you spec your enzyme, format, or bulk quantity.

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